ABSTRACT
Objective: To investigate the expression of long non-coding RNA(lncRNA) SNHG3 in esophageal squamous cell carcinoma (ESCC) and its effect on the migration and invasion of ECA-109 cells. Methods: Samples of tumor and corresponding para-carcinoma tissues were collected from 60 patients with ESCC, who were enrolled in Anyang Tumor Hospital from June 2011 to June 2014. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of lncRNA SNHG3 in ESCC tumor and para-carcinoma tissues, ECA-109 ESCC cells, and HEEC human normal esophageal epithelial cells. The correlation between lncRNA SNHG3 expression and clinical characteristics of patients with ESCC was assessed. ECA-109 cells were transfected with siRNA-SNHG3 plasmids to knockdown lncRNA SNHG3 expression. The correlation between lncRNA SNHG3 expression and patient prognosis was determined by Kaplan-Meier analysis. Transwell assay was used to investigate the migration and invasion abilities of ECA-109 cells. SNAIL and TWIST mRNA and protein levels in ECA-109 cells were detected by qRT-PCR and Western blot assay, respectively. Results: The expression level of lncRNA SNHG3 was significantly higher in ESCC tumor tissues compared to that in pericarcinomatous tissues and in ECA-109 cells compared to that in HEEC cells (both P<0.05). LncRNA SNHG3 expression in ESCC cells was significantly correlated with tumor differentiation, TNM stage, and lymph node metastasis (P< 0.05). LncRNA SNHG3 expression in ECA-109 cells decreased significantly upon transfection with the siRNA-SNHG3 plasmid (P<0.05). High expression of lncRNA SNHG3 was significantly correlated with poor prognosis in patients with ESCC. After lncRNA SNHG3 knockdown, the migration and invasion of ECA-109 cells and the mRNA and protein expression levels of SNAIL and TWIST were reduced significantly (P<0.05). Conclusions: LncRNA SNHG3 is highly expressed in ESCC tumor tissues and cells and promotes the migration and invasion of ECA-109 cells by regulating the expression of SNAIL and TWIST proteins.
ABSTRACT
Increasingly, researches have shown that long non-coding RNA (lncRNA) plays an important role in the oncogenesis and development of various tumors. Small nucleoli RNA host gene 3 (SNHG3) is a newly discovered lncRNA whose abnormally high expression is closely related to the overall survival and prognosis of tumor patients. SNHG3 can regulate the oncogenesis and development of tumors by endogenous competitive adsorption of miRNA, regulating cell cycle, mediating epithelial and mesenchymal transformation, and activating multiple signaling pathways. Therefore, in-depth research on the carcinogenesis mechanism of SNHG3 is helpful for early diagnosis, targeted therapy and prognostic assessment of relevant tumors. This paper reviews latest research progress on the expression and mechanism of SNHG3 in breast cancer, ovarian cancer, kidney cancer, liver cancer, stomach cancer, colon cancer, osteosarcoma and head and neck tumors to provide references for future studies.
ABSTRACT
Increasingly, researches have shown that long non-coding RNA (lncRNA) plays an important role in the oncogenesis and development of various tumors. Small nucleoli RNA host gene 3 (SNHG3) is a newly discovered lncRNA whose abnormally high expression is closely related to the overall survival and prognosis of tumor patients. SNHG3 can regulate the oncogenesis and development of tumors by endogenous competitive adsorption of miRNA, regulating cell cycle, mediating epithelial and mesenchymal transformation, and activating multiple signaling pathways. Therefore, in-depth research on the carcinogenesis mechanism of SNHG3 is helpful for early diagnosis, targeted therapy and prognostic assessment of relevant tumors. This paper reviews latest research progress on the expression and mechanism of SNHG3 in breast cancer, ovarian cancer, kidney cancer, liver cancer, stomach cancer, colon cancer, osteosarcoma and head and neck tumors to provide references for future studies.